Cloning PCR products

Dr. N.A. Affara naffara at uk.ac.crc
Wed Jun 3 05:48:54 EST 1992


Has anyone tried to cut an ordinary fragment of DNA with an enzyme 
that is close to the end. I have been wondering if PCR products don't
cut because the site is near the end or just something else. 
I am also trying a way to clone PCR products without cutting the 
product first. (at the moment this is untested).
Thanks for any help.



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