Cloning PCR products

Dr. N.A. Affara naffara at
Wed Jun 3 11:03:05 EST 1992

This is a repeat of an earlier message which I think didn't get through,
I apologise if you have seen this before.
Has anyone tried to cut a DNA fragment (not a PCR product) with an enzyme
which is near to the end e.g. up to 25 bases away. I am interested to
find out if the problem cutting PCR products is because the site is too
close to the end or something else entirely.


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