PCR re-amplification

Richard Sucgang rs54 at cunixf.cc.columbia.edu
Tue Jun 2 21:26:04 EST 1992


In article <1992Jun2.053705.14020 at talon.ucs.orst.edu> listona at bionette.cgrb.orst.edu (Aaron Liston) writes:
>I would like to hear from anyone who is successfully re-amplifying
>PCR products.  How do you do it?
>Thanks.

Well, you dilute your product 100 fold, and stick it back into a fresh
mixture with buffer and dNTP's and primers.

personally, unless you have already sequenced and confirmed that the pro-
duct is specific, I wouldn't reamplify, because artifacts at this stage all
have perfect matches to the target, and will amplify as readily. 
Opinions from the rest of the net?


>
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++Aaron Liston                                    E-mail: LISTONA at CGRB.ORST.EDU
>Department of Botany and Plant Pathology        Tel: 503 737 5301
>Oregon State University                         Fax: 503 737 3479
>Corvallis, OR 97331-2902
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++


-rich
Richard Sucgang : Dept. of Anatomy and Cell Biology
Columbia University (sucgang at cuhhca.hhmi.columbia.edu; 
de slime god         rs54 at cunixf.cc.columbia.edu)



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