rs54 at cunixf.cc.columbia.edu
Tue Jun 2 21:26:04 EST 1992
In article <1992Jun2.053705.14020 at talon.ucs.orst.edu> listona at bionette.cgrb.orst.edu (Aaron Liston) writes:
>I would like to hear from anyone who is successfully re-amplifying
>PCR products. How do you do it?
Well, you dilute your product 100 fold, and stick it back into a fresh
mixture with buffer and dNTP's and primers.
personally, unless you have already sequenced and confirmed that the pro-
duct is specific, I wouldn't reamplify, because artifacts at this stage all
have perfect matches to the target, and will amplify as readily.
Opinions from the rest of the net?
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++Aaron Liston E-mail: LISTONA at CGRB.ORST.EDU
>Department of Botany and Plant Pathology Tel: 503 737 5301
>Oregon State University Fax: 503 737 3479
>Corvallis, OR 97331-2902
Richard Sucgang : Dept. of Anatomy and Cell Biology
Columbia University (sucgang at cuhhca.hhmi.columbia.edu;
de slime god rs54 at cunixf.cc.columbia.edu)
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