PCR re-amplification

Stuart Brown browns at ccu.umanitoba.ca
Tue Jun 2 11:19:34 EST 1992


In article <1992Jun2.053705.14020 at talon.ucs.orst.edu> listona at bionette.cgrb.orst.edu (Aaron Liston) writes:
>I would like to hear from anyone who is successfully re-amplifying
>PCR products.  How do you do it?
>Thanks.
>
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++Aaron Liston                                    E-mail: LISTONA at CGRB.ORST.EDU
>Department of Botany and Plant Pathology        Tel: 503 737 5301
>Oregon State University                         Fax: 503 737 3479
>Corvallis, OR 97331-2902
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

I'm not sure I know what you mean by re-amplification.  If you mean taking some
PCR product and putting it back into another PCR reaction to get more product,
then I have done it.  It's really easy- just isolate your product by a column
or from a gel, then put a tiny, tiny, tiny bit (maybe 1:10^8 dilution) as
template into a new reaction.  Be very, very careful not to contaminate your
PCR pipetts and reagents with PCR product or else you may never make any other
PCR products in your lab again.
	-Stu

-- 
Stuart M. Brown                               Postdocs rush in where wise men
U. of Manitoba, Dept. Plant Science, Canada       Fear to tread. 
browns at ccu.umanitoba.ca        
				Disclaimer?? Sure: "That Disc is Mine!"



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