reamplification of PCR

Mike Poidinger skspoidn at
Tue Jun 2 14:09:32 EST 1992

In reply to 

>I would like to hear from anyone who is successfully re-amplifying
>PCR products.  How do you do it?

>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++Aaron Liston              >                      E-mail: LISTONA at CGRB.ORST.EDU
>Department of Botany and Plant Pathology        Tel: 503 737 5301
>Oregon State University                         Fax: 503 737 3479
>Corvallis, OR 97331-2902

I simply purify the PCR product (800 bp) by electrophoresis/gene clean, then use 1-5% of that
product in a fresh PCR reaction. This procedure generally works fine

Mike Poidinger
Dept of Microbiology
University of Reading

More information about the Methods mailing list