reamplification of PCR

Mike Poidinger skspoidn at uk.ac.rdg.susssys1
Tue Jun 2 14:09:32 EST 1992


In reply to 

>I would like to hear from anyone who is successfully re-amplifying
>PCR products.  How do you do it?
>Thanks.

>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++Aaron Liston              >                      E-mail: LISTONA at CGRB.ORST.EDU
>Department of Botany and Plant Pathology        Tel: 503 737 5301
>Oregon State University                         Fax: 503 737 3479
>Corvallis, OR 97331-2902
>++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

I simply purify the PCR product (800 bp) by electrophoresis/gene clean, then use 1-5% of that
product in a fresh PCR reaction. This procedure generally works fine

Mike Poidinger
Dept of Microbiology
University of Reading



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