Alkal. Phosphat. Buffers - Another Question !!!

PC386 at IL.AC.TAU.VE PC386 at IL.AC.TAU.VE
Mon Jun 1 06:44:09 EST 1992


Dear Friends on the net.

Some traffic went through the net regarding Alkaline Phosphatase buffers for
Western Blotting.
Many answers quoted the same buffer, and another mentioned the pre-made
buffer supplied by Promega.

Would anyone out there happend to know if these buffers are also appropriate
for use on immunohistochemistry slides (where I use ALP conjugated avidin...)?
If you do. Will you please append to your letter an established protocol
(from the ALP-Avidin stage onwards only...).

Thanks in advance :-)=

 **************************************************************
* Benny Shomer                                                 *
* Tel-Aviv University                                          *
* Sackler School of Medicine, Dept.of Embryology and Teratology*
*--------------------------------------------------------------*
* Snail:  Sheba Medical Center, Tel-Hashomer, 52625,   Israel. *
* E-mail :  PC386 at VE.TAU.AC.IL                                 *
* Tel :  972-3-640-9238     FAX :   972-3-642-2046             *
*                                   (c/o Dr. R. Ehrlich)       *
*                                                              *
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