Thermal cycler sequecing...

ww40 William_D_WARREN at UMAIL.UMD.EDU
Tue Mar 31 16:13:00 EST 1992


Does anyone out there get good results from thermal cycler sequencing ?

We have been trying to sequence gel isolated PCR products using
a gel purified end labelled primer (one of the PCR primers) and
can't get clean sequence. We've tried 3 step and two step cycling
with various time regimes and temperatures and still no luck.

Has anyone had better luck using this technique ???






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