single-stranded PCR

Ernest Retzel 1535 49118 ernest at lenti.med.umn.edu
Mon Mar 30 09:28:54 EST 1992


> We would greatly appreciate receiving a protocol for using PCR to make single
> stranded molecules. We thought about lambda exonuclease using one kinased 
> oligo to digest away one strand. Any ideas? Any suggestions. Where to get 
> lambda exonuclease from?

Why not put a biotin on one of your primers, and fish it out after the
PCR with magnetic beads?  This has been done for a variety of situations
already.

Ernest Retzel
Dept. of Microbiology
Univ. of Minnesota

ernest at lenti.med.umn.edu



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