Mark S. Whitsitt, N5RJF
msw1633 at rigel.tamu.edu
Mon Mar 30 15:50:00 EST 1992
In article <1992Mar30.142854.9212 at news2.cis.umn.edu>, ernest at lenti.med.umn.edu (Ernest Retzel (1535 49118)) writes...
>Why not put a biotin on one of your primers, and fish it out after the
>PCR with magnetic beads? This has been done for a variety of situations
>Dept. of Microbiology
>Univ. of Minnesota
How about a reference or two on that. I am not sure about how to biotinylate
the primer, ie how many biotins per primer, how much does the biotin affect the
PCR reaction , and how do you determine how many/much of the magnetic particles
do you add per estimated biotin content?
Lots of questions huh?
Thanks in advance
Mark S. Whitsitt, N5RJF Texas A&M University, Dept of Biochemistry
Vice-President W5AC ARC College Station, Tx. 77843-2128
Internet: MSW1633 at SIGMA.TAMU.EDU (409) 845-0832
Packet/APLINK: N5RJF @ KE5HE.TX.USA.NA
"You can't throw darts when you're empty, man." - another Shadelism
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