Blunt ended PCR products?

Dr. S.A.J.R. Aparicio saparici at crc.ac.uk
Fri Mar 27 18:50:47 EST 1992


I presume that you want your blunt end product for 
cloning? We routinely blunt end simply by adding T4 polymerase to the PCR mix and topping up with 2mM 
deoxy nucleotides. Actually I don;t blunt end PCR products on the whole but clone them with a home
made T-vector


Sam Aparicio
MRC Molecular Genetics Unit
Cambridge
UK



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