Microtubule depolymerisation

David Micklem DRM21 at MB1.BIO.CAM.AC.UK
Thu Mar 26 09:06:00 EST 1992


Dear Net,
	I am making protein extracts from fruit fly ovaries and need to
cause depolymerisation of the microtubules.  Does anyone know whether this
is possible to do by changing the buffer conditions, eg by including GDP or
metal ion chelators?  Id like to avoid using particularly stringent
conditions like high salt/detergent levels if possible.
Many thanks for your help.

_______________________________________________________________
D.R. Micklem                  :   "Time flies like an arrow...
Wellcome/CRC Institute        :
Cambridge                     :    Fruit flies like a banana"
DRM21 at MB1.BIO.CAM.AC.UK       :
_______________________________________________________________



More information about the Methods mailing list