Sequence for Okayama-Berg Vector?

aarment at desire.wright.edu aarment at desire.wright.edu
Mon Mar 23 14:18:15 EST 1992


	One of my fellow students is experiencing trouble PCRing a fragment
isolated from an Okayama-Berg Vector.  I have been unable to find a sequence
for the vector to determine restriction sites (in the off-chance that she
isolated a vector fragment having the same size as her insert).  Does anyone
have a sequence map of this vector?
	Also, all of her PCR runs have what looks like a RNA bleb on the gel,
but no bands.  Any clues as to what else might be wrong if the vector turns
out to not fit the bill?


aarment at desire.bitnet

 



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