making Taq polymerase
num208jn at MBDS.NRC.CA
Thu Mar 19 07:25:39 EST 1992
In article <9203181521.AA11052 at geneman.wustl.edu>, rick at GENEMAN.WUSTL.EDU (Rick Wilson) writes:
>>The original purification method for Taq DNA polymerase is:
>>Chien, A., Edgar, D.B. & Trela, J.M. (1976) "Deoxyribonucleic
>>acid polymerase from the extreme threomphile Thermus aquaticus"
>>J. Bacteriol. 127:1550-1557
>>A three step method: DEAE-Sephadex, Phosphocellulose & DNA cellulose.
>Beware! Hoffman-LaRoche is going after those labs attempting to do this!
>(Speaking from personal experience)
If you are not constrained not to (neat! a double negative!), could
you elaborate, please. I though that if you followed published
procedures and didn't try and sell it, you could extract it. Are they
going after PROMEGA, BRL and the rest?
Dr. John Nash, |
Cell Systems Section, | Email: Jo.Nash at NRC.CA
Institute for Biological Sciences, | Mail to "NUM208JN at MBDS.NRC.CA"
National Research Council of Canada, | is forwarded to the first address
Ottawa, Ontario, Canada. | automatically.
==> Disclaimer: All opinions are mine, not NRC's! <==
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