making Taq polymerase

Rick Wilson rick at GENEMAN.WUSTL.EDU
Wed Mar 18 10:21:39 EST 1992


>The original purification method for Taq DNA polymerase is:
>Chien, A., Edgar, D.B. & Trela, J.M. (1976) "Deoxyribonucleic
>acid polymerase from the extreme threomphile Thermus aquaticus"
>J. Bacteriol. 127:1550-1557
>A three step method: DEAE-Sephadex, Phosphocellulose & DNA cellulose.
>Good Luck

Beware!  Hoffman-LaRoche is going after those labs attempting to do this!
(Speaking from personal experience)
R. Wilson
St. Louis




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