making Taq polymerase

BACKD at QUCDN.QueensU.CA BACKD at QUCDN.QueensU.CA
Tue Mar 17 22:29:53 EST 1992


The original purification method for Taq DNA polymerase is:
Chien, A., Edgar, D.B. & Trela, J.M. (1976) "Deoxyribonucleic
acid polymerase from the extreme threomphile Thermus aquaticus"
J. Bacteriol. 127:1550-1557
A three step method: DEAE-Sephadex, Phosphocellulose & DNA cellulose.
Good Luck
Don Back
Dept of Biochem.
Queen's University
Kingston, Ontario



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