DNA synthesizers

simpson at biovx1.DNET.NASA.GOV simpson at biovx1.DNET.NASA.GOV
Tue Mar 10 20:27:36 EST 1992

Has anyone out there encountered a problem with DNA synthesis using
the ABS 292 (2 column) machine in which wrong bases get incorporated?
My colleague tells me that he gets 60% of the oligos (40 mers) with 
at least 1 base substitution and sometimes a deletion within the sequence. 
This is without PCR, just cloning and sequencing. 

Larry Simpson
UCLA Biology Department

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