dot blot suggestions
Cheung C. Yue
ccy at po.CWRU.Edu
Sat Mar 7 10:49:34 EST 1992
I am doing some recombinant baculovirus screening using the method of
limiting dilution and dot blotting. Unfortunately I am trying to do
3 at a time, and my Bio-Rad dot blot apparatus is extremely slow.
My questions are:
1. Do folks have other favorite 96-well dot blot apparatus which may
work more smoothly? Has anyone tried the new Gibco 96 well
dot blot apparatus with the 6 mm dot size (which does not use
any clamps)?
2. Should I be using a vacuum pump instead of house vacuum?
3. Any other method to transfer 96 samples onto a nitrocellulose
membrane?
Any suggestions are welcomed.
ccy at po.cwru.edu
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