Getting rid of E.c. RNA polymerase

misra at sask.usask.ca misra at sask.usask.ca
Tue Mar 3 15:48:12 EST 1992


In article <1992Feb29.001131.8355 at usenet.ins.cwru.edu>, bl275 at cleveland.Freenet.Edu (Dan Diaz) writes:
> 
> I am very close to getting some very pure cloned exo VII 
> holoenzyme from E. coli.  I have two bands near the top of the
> gel, which someone has suggested look like the beta and beta
> prime subunits of RNA polymerase, indicating that I have some
> RNAP core hanging around.
> 
> I would appreciate suggestions for an easy way to get rid of this
> contaminant, on the assumption that it is RNAP core.  I was
> thinking about using an ATP affinity column or some dye column
> which would bind the polymerase and let my exonuclease pass
> through.  If anyone has any bright ideas, please email them over.
> -- 
> Dizzy Dan Diaz  ddiaz at cwru.bitnet  bl275 at cleveland.freenet.edu
> Department of Biochemistry and Leisure  CWRU School of Medicine
> "If God had meant for us to get up early, sunrise would be noisy"



I am looking for a public domain program called Mcplasmap.

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