Bind silane protocols for seq gels [A Procedure]
Bill Melchior, NCTR/FDA
wmelchior at NTDOC.NCTNET.GOV
Mon Mar 9 09:57:37 EST 1992
>This is protocol is of extreme interest to me since I was about to jump off
>the deep end and try drying a sequencing gel without benefit of a gel drier
>tommorrow. The protocol I was going to follow though was published in
>Biotechniques 11(4):473 by Aaron Jastrow. He describes the use of a "Curl
>Has anyone tried this? I'm going to try it on a test gel at least.
I've not tried a hair dryer. I now use a large oven, set to 110 deg C.
An internal fan to circulate the air speeds drying; I usually go about 1-1/4
hours. Before it was available, I placed the glass plate with the gel on it
on an aluminum plate suspended about 1/2-1 cm above a hot plate. The only
problem with this is that's its easy to get the metal too hot, causing the
glass plate to crack; the oven is easier to use safely. Since the fixing
soak is so short I've always used it. (I've read that it's more important
to remove the urea for 35-S than for 32-P.)
Note that Jastrow says he dries "until the gel is no longer sticky to the
touch". In my experience, that's not a reliable criterion. I've had the
gel seem dry on the surface, but evidently still be damp inside, since the
film has sometimes stuck to the gel after overnight exposure. I assume that
internal moisture slowly diffuses to the temporarily dry surface.
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