Dissociating cell sheets
adavies at vax.oxford.ac.uk
Fri May 29 03:00:33 EST 1992
In article <+74k!hc at lynx.unm.edu>, kim at m44.unm.edu writes:
> Hi group:
> Does anyone have a way of making single cell suspensions of cell monolayers
> that does not trash extracellular protein markers? Since she is going to be
> using a multiparameter flow analysis, this has to be done by flow rather than
> using something like a Western or RIA.
Gently policeman the cells of TC plastic (commercial scrapers, eg Costar, are
best) and massage them through a sterile cell strainer (eg Falcon 35 2350).
This works really well for tightly clumped and/or adherent cells which would
otherwise be impossible to FACS.
Anthony Davies Voice: 865-512361
Institute of Virology Fax: 865-59962
Mansfield Road Vax: adavies%uk.ac.oxford.vax
Oxford OX1 3SR
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