stable transfection

Fiberman wsun at jeeves.ucsd.edu
Sun Nov 8 17:30:41 EST 1992


Hello Netter,

I am going to use the pRc/CMV vector from Invitrogen to express a cDNA
in HELA cells.  I want to select for the transfected cells with
neomycin resistance and establish a stably transfected cell line
expressing my clone.  Can anyone out there recommend a good reference,
or give some helpful tips on how to proceed?  Any suggestions
would be appreciated.

-fm



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