Which salt of X-P to use for detection of alkaline phosphatase?

Basavaraju Shankarappa bsh at MED.PITT.EDU
Thu Nov 12 11:02:59 EST 1992


We used to use the following as substrates for Western blotting.
Yields a bright pinkish band which quite pleasing to the eye and slide-
making but may be not that high contrast for the photography.

100 mg Fast Red TR salt (Sigma: f8764)
50 mg Napthol AS-MX phosphate (Sigma N5000)
              ^^^^^^^^^^^^^^^
Each of these are dissolved in 50 ml of 0.5M tris pH 8.0, mixed in the 
tray and incubated with the membrane. 

Worked well for me.
Raj Shankarappa
bsh at med.pitt.edu



More information about the Methods mailing list