Wanted: practical experience with Gene Sprinter
Cheung C. Yue
ccy at po.CWRU.Edu
Sun Nov 15 10:50:29 EST 1992
I saw the video from Fotodyne on their new sequencing package called
Gene Sprinter. It basically uses 50 micron thick acylamide gels run
in a controlled-temperature apparatus using a 10,000 volt power supply.
Gel runs are typically 30 minutes or so.
Gels are dried right on the glass plate by a simple heating device with
no need for vacuum. Volumes of running buffers used amount to less than
100 or so ml. per run. The gels are a bit short so they claim readings
of 250 bp or so. From my perspective, this apparatus seems ideal for
many of the shorter sequencing runs one needs in a mol biol lab. The
price tag for the whole package amounts to $10,000 or so. They told
me they would not sell any components of the systems separately (kind
of wasteful in my case since I have 2 recirculating pumps in the lab
already). I am curious if anyone on the net have had direct practical
experience with this system, and whether things are as easy and convenient
as they seem in the demo.
I have no connections with Fotodyne. I am merely interested in feedback
on this system.
ccy at po.cwru.edu
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