Live Bgal detection

Keith Robison robison1 at husc10.harvard.edu
Thu Nov 19 10:50:54 EST 1992


>Hey all! I wonder if anyone can recommend a way of detecting 
>B-galactosidase activity in living eukaryotic tissue or cells
>without having to fix them? Well, if you can't think of one, how
>about a different reporter gene that can be detected in live
>tissue? 

Look in Molecular Bioprobes' catalog -- they have a number of
substrates for a number of enzymes (including lacZ) which can
be used in living tissue.  Luciferases are another option --
either the firefly or bacterial ones (which use different
substrates).



Keith Robison
Harvard University
Department of Cellular & Developmental Biology
Department of Genetics / HHMI

robison at biosun.harvard.edu 



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