Removing dye from 373A reactions

scarr at kean.ucs.mun.ca scarr at kean.ucs.mun.ca
Thu Nov 19 14:01:32 EST 1992


Has anyone found a cheap, quick, reliable way of removing excess dye
terminators from a Cycle Sequencing reaction for an ABI373A sequencer?
We usually use an isopropanol extraction, but this seems to leave
variable amounts of the dyes behind. The ABI recommended BioSpin columns
do a better job, but they are costly and the ritual is slow & tedious.
Anyone had any experience with other commerical columns?

Thanks for the input

Steve Carr 



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