Result from "Primer" programme
biosmd at luxor.latrobe.edu.au
Sat Nov 21 22:59:31 EST 1992
I have been using the "Primer" programme on the published sequence of a mouse
gene (HSP70.1) and it reports homology in the 3' non-transcribed region, with
the mouse repetitve element B2.
My question is, could this explain why my attempts at using a probe which
is derived from the 3' region of this gene, has failed so far in Southern
blots - all I get is hybridization to apparently all DNA, even when washed
at 65 degrees in 0.1xSSC.
Does anyone know HOW homolgous the sequence has to be before Primer reports
Any comments appreciated,
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