Result from "Primer" programme

Sean Davidson biosmd at luxor.latrobe.edu.au
Sat Nov 21 22:59:31 EST 1992


I have been using the "Primer" programme on the published sequence of a mouse
gene (HSP70.1) and it reports homology in the 3' non-transcribed region, with
the mouse repetitve element B2.
My question is, could this explain why my attempts at using a probe which
is derived from the 3' region of this gene, has failed so far in Southern
blots - all I get is hybridization to apparently all DNA, even when washed
at 65 degrees in 0.1xSSC.
Does anyone know HOW homolgous the sequence has to be before Primer reports
it ?

Any comments appreciated,
Sean



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