deblocking

adavies at vax.path.ox.ac.uk adavies at vax.path.ox.ac.uk
Fri Nov 20 04:42:36 EST 1992


In article <19NOV92.16440280 at wums.wustl.edu>, wetsel_r at wums.wustl.edu writes:
> I assume that the oligo has been deprotected and off the column?  If so, I 
> would assume that they are currently in NH4-OH.  Deprotection is then easy. 
> We have our oligos in 4ml Wheaton vials available from VWR/Fisher which fit 
> nicely into a heating block.  Heat them overnight at 60'C.   It can be done 
> in an eppendorf tube as well, in fact there is probably no reason why 
> deprotection couldn't be done in a 60'C water bath as long as absolute care 
> is taken not to get any water mixed into the oligo.

Yep, we do just this.

Anthony Davies
Institute of Virology
Oxford UK



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