PCR with TMACL
shecht at lamar.ColoState.EDU
Wed Nov 25 15:00:11 EST 1992
I just can't find the two papers I have on this, but I will mention
that the USB catalog mentions this use of TMACl and has a graph of
the oligo length and Tm. in TMACl. (they also published in their
winter 91 COMMENTS vol 18, no.3, a very nice summary of enhancement and
specificity PCR approaches, comparing and explaining hot start, SSB, and
T4 gene 32).
I have used TMAC in PCR and found it did help some in my degenerate (primers,
that is) system but not enough to continue. Their SSB version did help a l
little bit as well. The previous replies regarding opt. are quite right too.
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