Help: In situ oligo probe design
warda at vax.oxford.ac.uk
warda at vax.oxford.ac.uk
Thu Nov 26 07:31:47 EST 1992
I'm about to start some non-radioactive (digoxigenin-labelled) in situ
hybridisations on mouse embryos. Is there any dogma on probe size using
oligodeoxynucleotides? There's a 37-bp sequence conserved between mouse
human and rabbit that looks suitable for the probe - should I get the full
37-mer synthesized, or would a smaller probe be just as effective? Also,
is secondary structure a consideration? (The putative probe sequence can
potentially form a hairpin loop with 6 complementary base pairs and a
5-base loop).
Thanks in advance for any help,
Bill Bennett JANET: warda at uk.ac.oxford.vax
CRC Growth Factors Research Group Internet: warda%vax.oxford.ac.uk
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