PCR with TMACL

Martin Kennedy cytogen at chmeds.ac.nz
Thu Nov 26 19:51:15 EST 1992


>  I just can't find the two papers I have on this, but I will mention
>  that the USB catalog mentions this use of TMACl and has a graph of
>  the oligo length and Tm. in TMACl. (they also published in their
>  winter 91 COMMENTS vol 18, no.3, a very nice summary of enhancement and 
>  specificity PCR approaches, comparing and explaining hot start, SSB, and 
>  T4 gene 32).
> 
>  I have used TMAC in PCR and found it did help some in my degenerate (primers,
>  that is) system but not enough to continue. Their SSB version did help a l
>  little bit as well. The previous replies regarding opt. are quite right too.
> 


Just for the record, and to save anyone else scrabbling through their
voluminous files of PCR methods,  here is the TMAC ref Sonya was looking for:

Hung et al. (1990).  NAR 18, 4953

Cheers,

Martin



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