Methods of detecting cellulose
MMELAN at PATTIE.WELLESLEY.EDU
Sun Nov 29 15:57:00 EST 1992
I have had great success in detecting cellulose (down to just a few
microfibrils) using the fluorescent dye Calcofluor white (now marketed by
Polysciences as Cellufluor). You will need to locate a fluorescence microscope
that has a UV excitation cube (365 nm excitation wavelength usually used for
Hoechst/DAPI DNA detection) check with the microscopists in your department.
If you only need a little of the dye (working conc. is 5ug/ml) I'd be happy
to send you some.
Melissa Melan mmelan at lucy.wellesley.edu
Department of Biological Sciences
Wellesley, MA 02181
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