protein biotinylation

[wyan at CUMC.CORNELL.EDU]

>
>Hi, everyone:
>
>    I am trying to screen a lamdaZAP library with a biotinylated GST-R ( R is 
>intracellular domain of a receptor) fusion protein, with the hope to find something downstream bind speceficly to the receptor. So far, all the "positive plaqes" I detected are also "positive " in the GST control.  Has anybody been lucky in the samilar approach ?  Any valuble suggestions on following points are greatly appreciated, e.g. protein biotinylation, buffer systerm used in protein-protein interaction.
>
>
>wei Yan
>
>Cornell University
>
>