whence Coomassie?

[BI1RGW at ibm.shef.ac.uk]

Hello Netland.

Can anyone remind me of the details of what, how and when Coomassie R/G bind
to proteins?  As I recall, R binds slower but is more sensitive - but what do
they bind?  I have at the back of my mind some vague inkling about lysine
residues - but what about arginine, and what about the N-terminus?

I'm sure this is a FAQ, but...all comments, recollections, anecdotes welcome.

Thanks in advance,


Robin Walters                      Robert Hill Institute
                                   University of Sheffield
BI1RGW at IBM.SHEF.AC.UK              P.O. Box 594
                                   Sheffield S10 2UH.
+44 (0)742 768555 X4244            UK