cycle seq. of lambda

Gessler Manfred Dr. gessler at convex.HRZ.Uni-Marburg.DE
Fri Oct 2 07:19:25 EST 1992


We would like to sequence lambda clones with cycle sequencing on an ABI
sequencer. Has anyone done this with dye-terminators, or do we need labelled
primers to get useful sequences? 
Any tips on protocols for DNA prep, oligo length etc. are welcome!

Thanks in advance

Manfred Gessler
Uni Marburg

gessler at convex.hrz.uni-marburg.de
FAX (49)6421-285630



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