PCR to prove transgenic plants

Jeremy Weinman jjw at rsbs0.anu.edu.au
Tue Oct 6 00:29:15 EST 1992


Hello fellow netters,
I'm after any info/hints/protocols/suggestions you might have regarding the 
PCR detection of transgenic plants.  We've used a binary Ti system using 
A. tumafaciens to (presumably) make the transgenic plants, but as they're 
onle small little plantlets so far, I was considering a PCR approach to 
testing for the presence of the intoduced construction. (Only 1 leaf 
needed).
Please send any ideas or experiences you've had with such methods to me and
I'll summarise to the group.
Thanxs in advance,
		Cheers,
			Jeremy
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr. Jeremy Weinman				Email:	jjw at rsbs1.anu.edu.au	
Plant Microbe Interaction Group			Phone:	61 6 2495051
Research School of Biological Sciences		Fax:	61 6 2490754
Australian National University			Snail:	PO Box 475, Canberra, 
							ACT 2601 AUSTRALIA



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