PCR max. lenght

[Klaus.Matthaei at anu.edu.au]

>Hi netters,
> 
>does anybody know the maximal lenght of the gene amplificable
>by PCR? I note, that we want to use it for the transfection
>into the eucaryotic cells.
> 
>Many thanks L.B.


G'Day

We have amplified 7kb and the literature suggests up to 15kb see:
 Kainz et al. Analytical Biochemistry 202,46-49 (1992) (but I haven't read
this paper closely).

Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

"If all else fails : Read the instructions"
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