PCR max. lenght

Klaus.Matthaei at anu.edu.au Klaus.Matthaei at anu.edu.au
Wed Oct 7 18:18:33 EST 1992

>Hi netters,
>does anybody know the maximal lenght of the gene amplificable
>by PCR? I note, that we want to use it for the transfection
>into the eucaryotic cells.
>Many thanks L.B.


We have amplified 7kb and the literature suggests up to 15kb see:
 Kainz et al. Analytical Biochemistry 202,46-49 (1992) (but I haven't read
this paper closely).

Cheers, Klaus
Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

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