hybridization problems

Sean Davidson s882076 at yallara.cs.rmit.OZ.AU
Fri Oct 9 22:11:10 EST 1992


Further to this discussion about different membranes, I recently began using
the Digoxigenin-based system for Northern blotting. Initial attempts used
Hybond N+, which is recommended for this procedure, but signal was always
virtually undetectable amongst high background. When I began using
Zetaprobe (Biorad) instead, results were (are) great.
  So do you think this may have just been the batch of Hybond, or why are
the results so much different?

Sean



More information about the Methods mailing list