hybridization problems

Sean Davidson s882076 at yallara.cs.rmit.OZ.AU
Fri Oct 9 22:11:10 EST 1992

Further to this discussion about different membranes, I recently began using
the Digoxigenin-based system for Northern blotting. Initial attempts used
Hybond N+, which is recommended for this procedure, but signal was always
virtually undetectable amongst high background. When I began using
Zetaprobe (Biorad) instead, results were (are) great.
  So do you think this may have just been the batch of Hybond, or why are
the results so much different?


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