PCR maximum length

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Sat Oct 10 16:05:49 EST 1992


:Rich Buckholz "no association with NEB" writes:

:In their recent (Sept. 92) publication "NEB Transcript" NEB claims to 
:have extended primers over 13kb.  They don't say whether they managed to 
:isolate a fragment from that extension.

Rumor has it that a group at Johns Hopkins has cloned a 20 kb DNA
fragment using PCR and "Hot-Tub" polymerase. Okay guys, C'mon,
Fess up!

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA



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