tightest promoter expression

Erik Boye eboye at radium.uio.no
Mon Oct 12 08:52:29 EST 1992

It seems to me that you have three options:
1. The more easily tac-like promoters used in Herman Bujard's expression
vectors. I believe the promoter contains more operators.
2. The arabinose promoter, which has been used to clone genes for 
relatively toxic gene products.
3. The lambda pL promoter, which may be the best repressed promoter of 
the three (with excess cI857 repressor present). However, you will need
a temperature upshift to induce transcription.

More information about the Methods mailing list