Protease Inhibitors

Melissa Melan MMELAN at LUCY.WELLESLEY.EDU
Wed Oct 14 12:16:00 EST 1992


	In response to Brian's reply concerning possible intracellular
proteolysis of fusion proteins:
	I found the article "Minimizing Proteolysis in Escherichia coli: GeneticSolutions"  by Susan Gottesman in Methods in Enzymology Vol.185 pp.119-129
to be most helpful.  Dr. Gottesman has produced several strains of Lon mutants
and is very cooperative in sharing these E.coli strains with others. See the
article for specific genotypes of each strain.  Hope that this is helpful.
===========================================================================
Melissa Melan
Department of Biological Sciences
Wellesley College
Wellesley, MA  02181
(617) 283-3137
============================================================================
P.S.  Dr Gottesman's address is:
	Laboratory of Molecular Biology
	National Cancer Institute
	NIH
	Bethesda, MD  20892   USA



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