ung E.coli strains

the End jgraham at bronze.ucs.indiana.edu
Thu Oct 15 10:18:53 EST 1992


In planning a chemical mutagenesis that will involve cytosine deamination,
I need an E. coli strain that will tolerate the presence of uridylate residues
in plasmid DNA, but will propagate them as A-T basepairs, rather than A-U pairs
We currenttly use CJ236 as a host which will introduce deoxyuridine. Are there
many ung only strains out there ? 

Any leads appreciated,


Jim
J. Graham
Biology and Chemistry Depts.
Indiana University -Bloomington



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