gentle radioiodination protocols

[Klaus v. Knoblauch]

In article <1992Oct12.090307.9414 at vax.oxford.ac.uk>, adavies at vax.oxford.ac.uk (Anthony Davies) writes:
> What is the best protocol around these days for radioiodinating a protein 
> _without_ disrupting its function too much (chloramine T, for instance, seems 
> too aggressive for this purpose)?
> 
Well, not quite from these days, but some years ago I used Bolton-Hunters-
Reagent (N-Succinimidyl 3-(4-hydroxy-5[125I]iodophenyl)propionate) to iodinate
Protein A which was subsequently used as a secondary antibody in immuno-
detection assays (western blots). The method was quite ok (as far as one can
say this about working with [125]I at all) and funktionally - well, it
funktioned in an immunological sense. But you may want to consider subsituting 
it with [35]S, if it is possible for you. BTW: The [125]I-labelling agent
as well as a [35]S labelling agent are both available from Amersham, so check
their catalog for ordering info (it might be available from others as well, 
but i at that time used theirs). 
finally, just to once again clear it up, I have worked only with the iodine  
reagent at that time only.

			have fun with it,
					Klaus
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>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
> Half a bee, philosophically, must ipse facto half not be. But can it be an >
> entire bee, if half the bee is not a bee, due to some ancient injury?      >
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>Monthy Phyton>>>>>>>>>>
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