Method for Anchored PCR/RACE

Martin Kennedy cytogen at
Sun Oct 18 17:14:27 EST 1992

In article <1992Oct15.143546.10299 at>, gchacko at (George W Chacko) writes:
> In article <1992Oct15.162328.123 at> cytogen at (Martin Kennedy) writes:
>> Finally, anchored PCR doesn't seem to work over regions much longer
>> than about 500bp, at least in my experience. Some PCR reactions show
>> a nice tight band, others a smear - 
> I've gotten tight bands upto 1 Kb using a 3' RACE system. I use 2 ug
> of total RNA (the quality of RNA appears to be the most critical
> factor IMO).           

Sorry - I meant 5' RACE doesn't seem to work over much more than 500bp, but
maybe someone has some comments about that.  I guess it is the efficiency of
the RT step that is critical here (and of course the RNA quality)


More information about the Methods mailing list