PCR without KCl

Dr. S. Bhattacharya sbhattac at crc.ac.uk
Mon Oct 19 10:30:46 EST 1992


Has anyone tried PCR without KCl in the buffer?  This has been recommended
for the amplification of longer DNA molecules >2Kb, but what modifications
if any, does one make to the annealing temperature? I am trying to PCR up 
a 2 Kb cDNA from a lambdagt11 library without much success.
Thanks Shoumo



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