Help with IODO-GEN reagent

C-Y Khoo cyk10 at cus.cam.ac.uk
Tue Oct 20 10:14:59 EST 1992


hi, this is in reference to the iodogen reagent problem:

my supervisor (Colin Dingwall) says:

1.	use plastic tubes
2.	use a gentle stream of nitrogen to blow off the chloroform, instead of
a vacuum
3.	the chloroform must be dry (use a fresh bottle of it)

two other factors: the reaction is dependent on the protein concentration
and the
amount of iodogen in the tube. for references see BBRC (1978) vol 80 pp
849-857 and
Biochemistry (1978) vol 17 pp 4807-4817)

hope this helps!

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Chong-Yee Khoo                             E-mail: cyk10 at cus.cam.ac.uk
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cyk10 at cy-mac.welc.cam.ac.uk
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