Single-strand rescue of phagemids

Klaus.Matthaei at Klaus.Matthaei at
Thu Oct 22 18:04:06 EST 1992

>Following on the recent thread about decreased yields of single-stranded DNA -
>what are the current "best" protocols for single-strand rescue of phagemids? I
>am interested in preparing sequencing quality and quantity of ss DNA and wonder
>whether anybody has improved on the standard methods. For M13 ss preps we
>restrict growth times to 6-8 hours for fear of rearrangements and deletions,
>but has anybody got any thoughts on overnight plasmid rescue?

We routinely rescue overnight with pTZ phagemids.
Cheers, Klaus
Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at

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