Single-strand rescue of phagemids

Klaus.Matthaei at anu.edu.au Klaus.Matthaei at anu.edu.au
Thu Oct 22 18:04:06 EST 1992


>Following on the recent thread about decreased yields of single-stranded DNA -
>what are the current "best" protocols for single-strand rescue of phagemids? I
>am interested in preparing sequencing quality and quantity of ss DNA and wonder
>whether anybody has improved on the standard methods. For M13 ss preps we
>restrict growth times to 6-8 hours for fear of rearrangements and deletions,
>but has anybody got any thoughts on overnight plasmid rescue?
>Cheers,
>George


We routinely rescue overnight with pTZ phagemids.
Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

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