choosing primer sequences for PCR

John Bishop bishopj at lupine
Fri Oct 23 14:09:13 EST 1992

How does one recognize (and avoid) sequences with secondary structure when  
choosing primer sequences for PCR?  I have heard there are computer  
programs which will perform this task.  Would someone point me to their  
source or send them to me?  Thanks


John Bishop
Department of Botany
University of Washington, Seattle
bishopj at

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