Non-templated nucleotide addition in PCR
bapr7 at syma.sussex.ac.uk
Mon Oct 26 06:35:58 EST 1992
I am making site directed mutagenesis using PCR and am worried about
non-templated nucleotide addition at the 3' end by TAQ polymerase.
Has anyone got any experience with Vent polymerase or any other
polymerase which does not have this non-templated nucleotide addition.
AFRC Institute of Plant Science Research
Nitrogen Fixation Laboratory
University of Sussex
Brighton BN1 9Q
e-mail : JANET :- jacobjp at uk.ac.afrc.nfl
EARN/BITNET :- jacobjp at nfl.afrc.ac.uk
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