Non-templated nucleotide addition in PCR

Jansen Jacob bapr7 at syma.sussex.ac.uk
Mon Oct 26 06:35:58 EST 1992


I am making site directed mutagenesis using PCR and am worried about
non-templated nucleotide addition at the 3' end by TAQ polymerase. 
Has anyone got any experience with Vent polymerase or any other
polymerase which does not have this non-templated nucleotide addition.

			Jansen Jacob
			AFRC Institute of Plant Science Research
			Nitrogen Fixation Laboratory
			University of Sussex
			Brighton BN1 9Q
			U.K.

e-mail : JANET     :- jacobjp at uk.ac.afrc.nfl
       EARN/BITNET :- jacobjp at nfl.afrc.ac.uk



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