choosing primer sequences for PCR

Dan Jacobson danj at
Mon Oct 26 09:03:21 EST 1992

In article <1992Oct23.190913.20603 at> bishopj at lupine (John Bishop) writes:
>How does one recognize (and avoid) sequences with secondary structure when  
>choosing primer sequences for PCR?  I have heard there are computer  
>programs which will perform this task.  Would someone point me to their  
>source or send them to me?  Thanks

I have posted a Primer-FAQ a couple times recently.  It describes
the freely available programs and how to get them.  I have sent the
FAQ to John and will send it to anybody who writes me a note, I'll
post it again if I get a mailbox full of requests.


Dan Jacobson

danj at

More information about the Methods mailing list