PCR from Lambda Libraries

Anderson, Roger F. rander at romeo.caltech.edu
Mon Oct 26 21:13:00 EST 1992


I have routinely just taken aliquots from 1 to 10 microL
tossed them into a standard PCR reaction (either TAQ or
vent) and I get amplification. The titre of the library
will have some considerable effect.
If 96 -94 C doesn't pop those buggers you have a problem.
I have even done repeats off of genomic DNA. So tryit.



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